Usage of Peptide Antigens for Antibody-Based BoNT Detection System

Objective: In the current study, we aimed to elicit a BoNT-specific immune response via using peptides designed by bioinformatics tools instead of intact native BoNT A toxin itself and demonstrate usage these anti-peptide antibodies in detection system. Methods: Synthetic epitopic regions specific LC, HN HC were selected as antigen B cell “Epitope Prediction Tools” form IEDB shown be on surface with software (Discovery Studio 4.0). Selected used mice immunizations interaction between developed was examined. Results: The at picogram levels per milliliter performed against three different (P1, P2, P3) mice. Conclusion: study shows that synthetic are least effective or toxoid for raising high-affinity toxins. addition, considering need quick diagnosis botulism and, already test systems which many experimental animals sacrificed, results necessity peptide immunogens reduce both number amount usage.